Ab 1-42 -induced electrophysiological changes in hippocampal cultures.
Background information: Alzheimer’s disease (AD) is a neurodegenerative disease characterized by an extracellular accumulation of neurotoxic amyloid beta peptide, a proteolytic product of amyloid precursor protein (APP). The accumulation of amyloid beta peptides in synapses causes synaptic degeneration and dysfunctional neuronal electrical activity. Ab1-42 peptides alter hippocampal neuronal cultures electrical activity which can be rescued by a reference item.
Cells: Rat embryonic hippocampal neurons.
Damage induction: 5 mM Ab1-42 for 24 and 48h.
Readout: MEA electrical parameters (spikes, number of bursts/network bursts, synchronicity…)
Positive control: Reference item.
Compound treatment: Compounds are added to cells 24h before the addition of Ab1-42.
Turnaround time: 2 months.