Background information: Alzheimer’s disease (AD) is a neurodegenerative disease characterized by an extracellular accumulation of neurotoxic amyloid beta peptide, a proteolytic product of amyloid precursor protein (APP). The accumulation of amyloid beta peptides in synapses causes synaptic degeneration and cellular apoptosis and death. NGF can inhibit amyloid beta peptides-induced apoptosis.

Cells: PC12, rat pheochromocytoma.

Damage induction: 20 mM Ab 1-42 for 48h.

Readout: Apoptosis

Positive control: NGF.

Compound treatment: Compounds are added to cells 24h before the addition of Ab1-42 .

Turnaround time: 1 month.