Tissue Binding assay

Aim: Screening of in vitro tissue binding of test compound in tissue homogenate from rodents, using Rapid Equilibrium Dialysis (RED).

Background information: Tissue binding determines the amount of free compound available at the action site. The extent of binding to tissue components influences the efficacy and/or toxicological effects of drugs.

Equilibrium dialysis is the most widely accepted method for assessing plasma and tissue binding as non-specific binding effects are minimized compared to other methods such as ultrafiltration.

The RED device consists of disposable inserts and a base plate. Each insert is made of two side-by-side chambers separated by a vertical cylinder of a semi-permeable dialysis membrane with minimal nonspecific binding. The system is allowed to equilibrate at 37ºC. The amount of test compound present in each compartment is quantified by UPLC-MS/MS.

Compound requirement: 150 µl of a 10 mM DMSO solution

Turnaround time: 1 week / 2 compounds

Experimental design:

  • Test compound concentration: 5 µM (variable on request)
  • Number of replicates: 3
  • Dilution factor of tissue homogenate: 5 (in saline phosphate buffer)
  • Equilibrium dialysis conditions: 37ºC for 4 h
  • Dilution with blank tissue homogenate (buffer sample) or buffer (tissue homogenate sample)
  • Stability in tissue homogenate checked in parallel
  • Analytical method: UPLC-MS/MS quantification of tissue and buffer samples after protein precipitation

We are proud to announce that Welab is partner of this new EU funded project.

In collaboration with 12 partners from 6 countries, Welab Barcelona is establishing the Antivirus Pandemic Preparedness EuropeAn pLatform (APPEAL), a European research initiative aimed at enhancing preparedness for future pandemics. This EU funded collaboration will establish a comprehensive program for the development of broad-spectrum antiviral drugs within a five year time frame ensuring drug affordability and accessibility to low income countries.
Link to the press release: https://lnkd.in/d-jmV2CS