WELAB BARCELONA

Tissue Kinetics assay in rodents

 

Aim: To characterize the pharmacokinetics of test compound in a tissue and plasma after single dose administration to rodents.

 

Background information: Tissue distribution studies provide information on distribution and accumulation of a drug at the site of action. This information may be useful for understanding results from toxicological or pharmacological studies.

Tissue pharmacokinetics in rodents is conducted after single dose administration of the compound by the selected route and vehicle. At time points defined previously, blood samples and tissue are collected from each animal. After tissue homogenization and protein precipitation, compound is determined in brain and plasma by UPLC-MS/MS.

 

Compound requirement: dependent on species, dose and route

Turnaround time: 3 weeks 

Experimental design:

Dose and route: variable on request (e.g. oral, intraperitoneal)
Vehicle: variable depending on route
Samples: plasma and tissue at each time (8 sampling times up to 24 h)
Replicates: 2 animals / sampling time
Dilution factor of tissue homogenate: 5 (in saline phosphate buffer)
Analytical method: UPLC-MS/MS quantification in plasma and tissue after protein precipitation using calibration curves

Delivered results:

Plasma concentration-time curve
Tissue concentration-time curve
Standard pharmacokinetic parameters in plasma and tissue determined by non-compartmental analysis
Tissue-to-plasma exposure ratio
tissue kinetics3