Glutamate excitotoxicity

Background information: Glutamate receptor overactivation greatly contributes to neuronal injury (excitotoxicity) mainly due to excessive stimulation of NMDA and AMPA receptors. Glutamate-induced excitotoxicity is a hallmark in most neurodegenerative diseases like multiple-sclerosis, Alzheimer’s disease or ALS, among others. Glutamate reduces cell viability in GT1/7 cells and MK-801 inhibits glutamate-induced cell death in a concentration-dependent manner.


Cells: GT1/7, mouse hypothalamic GnRH Neuronal.

Damage induction: 2.5 mM glutamate for 96h.

Readout: Cell viability.

Positive control: MK-801.

Compound treatment: Compounds are added to cells 24h before the addition of glutamate.

Turnaround time: 45 days.