MPP+ neurotoxicity in SH-SY5Y cells

Background information: The neurotoxin 1-methyl-4-phenyl pyridinium (MPP+) enters neurons through DAT (Dopamine transporter) causing specific dopaminergic neuronal apoptosis and death, providing a useful model to study Parkinson’s disease in vitro. MPP+ induces apoptosis in SH-SY5Y cells. SB-203580 is able to inhibit MPP+-induced apoptosis


Cells: SH-SY5Y, human neuroblastoma.

Damage induction: 3 mM MPP+ for 24h.

Readout: Apoptosis.

Positive control: SB-203580.

Compound treatment: Compounds are added to cells 24h before the addition of MPP+.

Turnaround time: 1 month.