Screening Pharmacokinetic assay in the rat
Aim: To characterize the pharmacokinetics of test compound in the rat after single oral or intravenous dose administration.
Background information: Pharmacokinetics is the study of changes in drug concentration over time as a result of the ADME procesess that occur in the body after drug administration (Absorption, Distribution, Metabolism and Excretion). During the Drug Discovery process, new synthetised candidates with an optimum pharmacokinetic profile in rodents are screened for further characterization.
Screening pharmacokinetics in the rat is conducted after single dose administration of the compound by the selected route and vehicle. At time points defined previously, serial blood samples are withdrawn from each animal. After centrifugation and protein precipitation, compound is determined in plasma by UPLC-MS/MS.
Compound requirement: 25 mg
Turnaround time: 3 weeks