Aim:
To evaluate the excretion of test compound in urine collected after administration to the rat by different routes.

Background information:The primary route in which the body eliminates substances is through the kidneys. Factors that influence renal excretion include plasma drug concentration, plasma protein binding and renal function. Drugs are transferred from plasma into the urine by glomerular filtration and active tubular secretion.

After administration of test compound to rats by the selected route (study-dependent) different urine fractions are collected at pre-set time intervals. Vehicle is previously administered and control urine (or basal) is collected. Samples are
quantified by UPLC-MS/MS at each interval using a calibration curve prepared in blank urine.