Renal excretion assay

To evaluate the excretion of test compound in urine collected after administration to the rat by different routes.


Background information:The primary route in which the body eliminates substances is through the kidneys. Factors that influence renal excretion include plasma drug concentration, plasma protein binding and renal function. Drugs are transferred from plasma into the urine by glomerular filtration and active tubular secretion.

After administration of test compound to rats by the selected route (study-dependent) different urine fractions are collected at pre-set time intervals. Vehicle is previously administered and control urine (or basal) is collected. Samples are
quantified by UPLC-MS/MS at each interval using a calibration curve prepared in blank urine.

Compound requirement: Dependent on route and dose selected

Turnaround time: 3 weeks / 1 compound


Experimental design:

Number of animals: 2 rats
Number of replicates: 2
Urine fractions collected: 0-6 h and 6-24 h
Analytical method: UPLC-MS/MS quantification


Delivered results:

Urine excretion of test compound expressed as percentage of bioavailable administered dose
Metabolite-to-parent compound concentration ratio (if metabolite standard is available)