WELAB BARCELONA

Pharmacokinetic assay in the dog

Aim:
To characterize the pharmacokinetics of test compound in the dog after single oral or intravenous dose administration.

 

Background information: Pharmacokinetics is the study of changes in drug concentration over
time as a result of the ADME procesess that occur in the body after drug administration (Absorption, Distribution, Metabolism and Excretion). The dog is one of the species included in the interspecies allometric scaling aimed at predicting the pharmacokinetics in humans. Drug exposure in the dog may also help with the dose selection for later toxicokinetic studies.

Pharmacokinetics in the dog is conducted after single dose administration of the compound by the selected route and vehicle. At time points defined previously, serial blood samples are withdrawn from each animal. After centrifugation, compound is determined in plasma by UPLC-MS/MS.

 

Compound requirement: 2 g

Experimental design:

–Administration and sample extraction is done in an external test facility
–Dose and route: 10 mg/kg (oral gavage) and 1 mg/kg (intravenous injection) (both variable on request) in the same animal after a 1-week washout period
–Animals: 4 dogs / route
–Vehicle: 0.5% methylcellulose (oral), variable for intravenous dosing
–Samples: serial blood sampling from each animal at preset times up to 72 h
–Analytical method: UPLC-MS/MS quantification in plasma after plasma protein precipitation or solid phase extraction using a calibration curve.

 

Delivered results:

–Plasma concentration-time curves
–Standard pharmacokinetic parameters determined by non-compartmental analysis

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